murine macrophages cell line ic21 Search Results


macrophages  (ATCC)
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ATCC macrophages
(A) Schematic overview of the transwell migration experimental methods. (B) Quantification of migrated IC-21 cells on underside of transwell insert after 18 h of incubation with OS-EVs at 10 2 , 10 4 , and 10 5 EVs per cell. Data are mean ± SD (n=5, technical replicates). Groups with statistically significant differences based on two-way ANOVA do not share the same letters.
Macrophages, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Schematic overview of the transwell migration experimental methods. (B) Quantification of migrated IC-21 cells on underside of transwell insert after 18 h of incubation with OS-EVs at 10 2 , 10 4 , and 10 5 EVs per cell. Data are mean ± SD (n=5, technical replicates). Groups with statistically significant differences based on two-way ANOVA do not share the same letters.

Journal: Advanced biology

Article Title: Extracellular vesicles from highly metastatic osteosarcoma cells induce pro-tumorigenic macrophage phenotypes

doi: 10.1002/adbi.202300577

Figure Lengend Snippet: (A) Schematic overview of the transwell migration experimental methods. (B) Quantification of migrated IC-21 cells on underside of transwell insert after 18 h of incubation with OS-EVs at 10 2 , 10 4 , and 10 5 EVs per cell. Data are mean ± SD (n=5, technical replicates). Groups with statistically significant differences based on two-way ANOVA do not share the same letters.

Article Snippet: IC-21 murine macrophages (ATCC, Manassas, VA) were cultured in RPMI 1640 medium (ATCC Formulation: L-glutamine, HEPES, sodium pyruvate, and high glucose) supplemented with FBS (10%, GenClone, El Cajon, CA) under standard culture conditions.

Techniques: Migration, Incubation

(A) Representative confocal images of IC-21 macrophages (red) treated with OS-EVs labelled with CFSE (green, denoted with white arrows) in serum-free media for 4 h. White boxes denote magnified inset locations. Scale bar = 50 μm. Quantification of pixels above green autofluorescent signal of cells treated with EVs isolated from K12 and K7M2 cells (B) and the same EVs also treated with an anti-CD47 antibody (+Ab) (C) . Data are mean ± SD (n=3, technical replicates). Groups with statistically significant differences based on two-way ANOVA do not share the same letters; ns denotes no significance.

Journal: Advanced biology

Article Title: Extracellular vesicles from highly metastatic osteosarcoma cells induce pro-tumorigenic macrophage phenotypes

doi: 10.1002/adbi.202300577

Figure Lengend Snippet: (A) Representative confocal images of IC-21 macrophages (red) treated with OS-EVs labelled with CFSE (green, denoted with white arrows) in serum-free media for 4 h. White boxes denote magnified inset locations. Scale bar = 50 μm. Quantification of pixels above green autofluorescent signal of cells treated with EVs isolated from K12 and K7M2 cells (B) and the same EVs also treated with an anti-CD47 antibody (+Ab) (C) . Data are mean ± SD (n=3, technical replicates). Groups with statistically significant differences based on two-way ANOVA do not share the same letters; ns denotes no significance.

Article Snippet: IC-21 murine macrophages (ATCC, Manassas, VA) were cultured in RPMI 1640 medium (ATCC Formulation: L-glutamine, HEPES, sodium pyruvate, and high glucose) supplemented with FBS (10%, GenClone, El Cajon, CA) under standard culture conditions.

Techniques: Isolation

(A) Representative confocal images of IC-21 macrophages treated with OS-EVs in serum-free media for 24 h, then labelled with Click-iT ® EdU; scale bar = 100 μm. Quantification of EdU-positive IC-21 cells following culture with OS-EVs in serum-free (B) and FBS-supplemented (C) media for 24 h. Control groups for both experiments consist of basal media under serum-free (SF) or FBS-supplemented (FBS) conditions. Data are mean ± SD (n=5, technical replicates). Groups with statistically significant differences based on one-way ANOVA do not share the same letters.

Journal: Advanced biology

Article Title: Extracellular vesicles from highly metastatic osteosarcoma cells induce pro-tumorigenic macrophage phenotypes

doi: 10.1002/adbi.202300577

Figure Lengend Snippet: (A) Representative confocal images of IC-21 macrophages treated with OS-EVs in serum-free media for 24 h, then labelled with Click-iT ® EdU; scale bar = 100 μm. Quantification of EdU-positive IC-21 cells following culture with OS-EVs in serum-free (B) and FBS-supplemented (C) media for 24 h. Control groups for both experiments consist of basal media under serum-free (SF) or FBS-supplemented (FBS) conditions. Data are mean ± SD (n=5, technical replicates). Groups with statistically significant differences based on one-way ANOVA do not share the same letters.

Article Snippet: IC-21 murine macrophages (ATCC, Manassas, VA) were cultured in RPMI 1640 medium (ATCC Formulation: L-glutamine, HEPES, sodium pyruvate, and high glucose) supplemented with FBS (10%, GenClone, El Cajon, CA) under standard culture conditions.

Techniques: Control

Flow cytometric analyses of IC-21 macrophages treated with OS-EVs for 24 h. Quantifications of (A) Live cells, (B) F4/80 + cells, (C) CD86 + iNOS + (M1) macrophages, and (D) CD86 − CD206 + Arg1 + (M2) macrophages. (E) Multiplex proteomic analysis of secreted immunomodulatory cytokines from OS-EV treated macrophages measured via Luminex. Inset numbers are mean (n=3, biological replicates) cytokine concentrations in pg mL −1 . Data are mean ± SD (n=3, biological replicates). Groups with statistically significant differences based on non-paired t-tests do not share the same letters. ns denotes no significance.

Journal: Advanced biology

Article Title: Extracellular vesicles from highly metastatic osteosarcoma cells induce pro-tumorigenic macrophage phenotypes

doi: 10.1002/adbi.202300577

Figure Lengend Snippet: Flow cytometric analyses of IC-21 macrophages treated with OS-EVs for 24 h. Quantifications of (A) Live cells, (B) F4/80 + cells, (C) CD86 + iNOS + (M1) macrophages, and (D) CD86 − CD206 + Arg1 + (M2) macrophages. (E) Multiplex proteomic analysis of secreted immunomodulatory cytokines from OS-EV treated macrophages measured via Luminex. Inset numbers are mean (n=3, biological replicates) cytokine concentrations in pg mL −1 . Data are mean ± SD (n=3, biological replicates). Groups with statistically significant differences based on non-paired t-tests do not share the same letters. ns denotes no significance.

Article Snippet: IC-21 murine macrophages (ATCC, Manassas, VA) were cultured in RPMI 1640 medium (ATCC Formulation: L-glutamine, HEPES, sodium pyruvate, and high glucose) supplemented with FBS (10%, GenClone, El Cajon, CA) under standard culture conditions.

Techniques: Isolation, Multiplex Assay, Luminex